Review



f nucleatum subsp fusiform strain atcc 51190  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    ATCC f nucleatum subsp fusiform strain atcc 51190
    F Nucleatum Subsp Fusiform Strain Atcc 51190, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+nucleatum+atcc+51190/pmc11937130-13-26-31?v=ATCC
    Average 94 stars, based on 53 article reviews
    f nucleatum subsp fusiform strain atcc 51190 - by Bioz Stars, 2026-07
    94/100 stars

    Images



    Similar Products

    f nucleatum subsp fusiform strain atcc 51190  (ATCC)
    94
    ATCC f nucleatum subsp fusiform strain atcc 51190
    F Nucleatum Subsp Fusiform Strain Atcc 51190, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+nucleatum+atcc+51190/pmc11937130-13-26-31?v=ATCC
    Average 94 stars, based on 1 article reviews
    f nucleatum subsp fusiform strain atcc 51190 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    f nucleatum subsp fusiforme atcc 51190 species  (ATCC)
    94
    ATCC f nucleatum subsp fusiforme atcc 51190 species
    Logistic regressions result for the main analysis: association between the oral pathogen composite and elevated depressive and anxiety symptoms. Modest (nonsignificant) association between the pathogen composite and elevated depressive symptoms ( N = 39), compared to minimal depressive symptoms ( N = 397), both in unadjusted ( p =0.0940) and adjusted ( p =0.0712) models. No association of the pathogen composite between participants with elevated ( N = 66) versus minimal ( N = 370) anxiety symptoms, both in unadjusted and adjusted models ( p > 0.05). Adjusted models control for confounders: age, SES, diet, lifestyle risk, obesity, and hypertension. The pathogen composite reflects the combined load of P. gingivalis , T. denticola , F. <t>nucleatum</t> subsp. vincentii ATCC 49256 , F. nucleatum subsp. vincentii 3_1_36A2 , F. nucleatum subsp. vincentii , F. nucleatum subsp. animalis 7_1 , F. nucleatum subsp. polymorphum , F. nucleatum subsp. animalis ATCC 51191, F. nucleatum subsp. animalis , and P. nigrescens ATCC 33563 . Error bars represent 95% CI. 95% CI, 95% confidence interval; OR, odds ratio estimate; SE, standard error; SES, socioconomic status.
    F Nucleatum Subsp Fusiforme Atcc 51190 Species, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+nucleatum+atcc+51190/pmc12303655-123-23-27?v=ATCC
    Average 94 stars, based on 1 article reviews
    f nucleatum subsp fusiforme atcc 51190 species - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    f nucleatum ssp fusiforme atcc 51190 fnf  (ATCC)
    94
    ATCC f nucleatum ssp fusiforme atcc 51190 fnf
    Comparisons of planktonic (p) and biofilm-grown (b) F . <t>nucleatum</t> (FN) stimulating antimicrobial responses in human neutrophils. (A–C) Quantification of overall ROS released shown as area under the curve (AUC). (D) Quantification of NET-DNA. (E–H) Quantification of cytokine and (I–J) antimicrobial enzyme release. Numbers of samples in each data set analysed are specified in <xref ref-type= Supplementary Table 1 . ns, not significant; *p<0.05; **p<0.01; ***p<0.001. (A, B, D, I, J) One-Way ANOVA with Tukey’s post hoc test, mean values and SD are shown. (C, E–H) Kruskal-Wallis test with Dunn’s post hoc test, values shown as median and interquartile range (IQR). " width="250" height="auto" />
    F Nucleatum Ssp Fusiforme Atcc 51190 Fnf, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+nucleatum+atcc+51190/pmc11499235-43-41-45?v=ATCC
    Average 94 stars, based on 1 article reviews
    f nucleatum ssp fusiforme atcc 51190 fnf - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    f nucleatum subsp fusiforme strain atcc 51190  (ATCC)
    94
    ATCC f nucleatum subsp fusiforme strain atcc 51190
    Comparisons of planktonic (p) and biofilm-grown (b) F . <t>nucleatum</t> (FN) stimulating antimicrobial responses in human neutrophils. (A–C) Quantification of overall ROS released shown as area under the curve (AUC). (D) Quantification of NET-DNA. (E–H) Quantification of cytokine and (I–J) antimicrobial enzyme release. Numbers of samples in each data set analysed are specified in <xref ref-type= Supplementary Table 1 . ns, not significant; *p<0.05; **p<0.01; ***p<0.001. (A, B, D, I, J) One-Way ANOVA with Tukey’s post hoc test, mean values and SD are shown. (C, E–H) Kruskal-Wallis test with Dunn’s post hoc test, values shown as median and interquartile range (IQR). " width="250" height="auto" />
    F Nucleatum Subsp Fusiforme Strain Atcc 51190, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+nucleatum+atcc+51190/pm38479393-160-33-38?v=ATCC
    Average 94 stars, based on 1 article reviews
    f nucleatum subsp fusiforme strain atcc 51190 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    f nucleatum atcc 51190  (ATCC)
    94
    ATCC f nucleatum atcc 51190
    Comparisons of planktonic (p) and biofilm-grown (b) F . <t>nucleatum</t> (FN) stimulating antimicrobial responses in human neutrophils. (A–C) Quantification of overall ROS released shown as area under the curve (AUC). (D) Quantification of NET-DNA. (E–H) Quantification of cytokine and (I–J) antimicrobial enzyme release. Numbers of samples in each data set analysed are specified in <xref ref-type= Supplementary Table 1 . ns, not significant; *p<0.05; **p<0.01; ***p<0.001. (A, B, D, I, J) One-Way ANOVA with Tukey’s post hoc test, mean values and SD are shown. (C, E–H) Kruskal-Wallis test with Dunn’s post hoc test, values shown as median and interquartile range (IQR). " width="250" height="auto" />
    F Nucleatum Atcc 51190, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+nucleatum+atcc+51190/pm38477807-67-29-31?v=ATCC
    Average 94 stars, based on 1 article reviews
    f nucleatum atcc 51190 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Logistic regressions result for the main analysis: association between the oral pathogen composite and elevated depressive and anxiety symptoms. Modest (nonsignificant) association between the pathogen composite and elevated depressive symptoms ( N = 39), compared to minimal depressive symptoms ( N = 397), both in unadjusted ( p =0.0940) and adjusted ( p =0.0712) models. No association of the pathogen composite between participants with elevated ( N = 66) versus minimal ( N = 370) anxiety symptoms, both in unadjusted and adjusted models ( p > 0.05). Adjusted models control for confounders: age, SES, diet, lifestyle risk, obesity, and hypertension. The pathogen composite reflects the combined load of P. gingivalis , T. denticola , F. nucleatum subsp. vincentii ATCC 49256 , F. nucleatum subsp. vincentii 3_1_36A2 , F. nucleatum subsp. vincentii , F. nucleatum subsp. animalis 7_1 , F. nucleatum subsp. polymorphum , F. nucleatum subsp. animalis ATCC 51191, F. nucleatum subsp. animalis , and P. nigrescens ATCC 33563 . Error bars represent 95% CI. 95% CI, 95% confidence interval; OR, odds ratio estimate; SE, standard error; SES, socioconomic status.

    Journal: Depression and Anxiety

    Article Title: Associations Between Oral Microbiota Pathogens and Elevated Depressive and Anxiety Symptoms in Men

    doi: 10.1155/da/9961595

    Figure Lengend Snippet: Logistic regressions result for the main analysis: association between the oral pathogen composite and elevated depressive and anxiety symptoms. Modest (nonsignificant) association between the pathogen composite and elevated depressive symptoms ( N = 39), compared to minimal depressive symptoms ( N = 397), both in unadjusted ( p =0.0940) and adjusted ( p =0.0712) models. No association of the pathogen composite between participants with elevated ( N = 66) versus minimal ( N = 370) anxiety symptoms, both in unadjusted and adjusted models ( p > 0.05). Adjusted models control for confounders: age, SES, diet, lifestyle risk, obesity, and hypertension. The pathogen composite reflects the combined load of P. gingivalis , T. denticola , F. nucleatum subsp. vincentii ATCC 49256 , F. nucleatum subsp. vincentii 3_1_36A2 , F. nucleatum subsp. vincentii , F. nucleatum subsp. animalis 7_1 , F. nucleatum subsp. polymorphum , F. nucleatum subsp. animalis ATCC 51191, F. nucleatum subsp. animalis , and P. nigrescens ATCC 33563 . Error bars represent 95% CI. 95% CI, 95% confidence interval; OR, odds ratio estimate; SE, standard error; SES, socioconomic status.

    Article Snippet: K. pneumoniae species were not included as no OTU assigned to them was detected; similarly, we did not include A. actinomycetemcomitans species and F. nucleatum subsp. fusiforme ATCC 51190 species as these were poorly detected in the samples and not positively correlated with the composite (details in Supporting Information Table ; correlation matrix in Supporting Information Figure ).

    Techniques: Control

    Logistic regressions result for taxa within the composite and elevated depressive symptoms. Significant positive associations between P. nigrescens , F. nucleatum subsp. vincentii , F. nucleatum subsp. animalis ATCC 51191 , F. nucleatum subsp. animalis , and elevated depressive symptoms ( N = 39), compared to minimal depressive symptoms ( N = 397), both in unadjusted and adjusted models (all p < 0.05). No evidence of associations with the other species. Adjusted models control for confounders: age, SES, diet, lifestyle risk, obesity, and hypertension. Error bars represent 95% CI. 95% CI, 95% confidence interval; OR, odds ratio estimate; SE, standard error; SES, socioconomic status. Significant p -values ( p < 0.05) are in bold.

    Journal: Depression and Anxiety

    Article Title: Associations Between Oral Microbiota Pathogens and Elevated Depressive and Anxiety Symptoms in Men

    doi: 10.1155/da/9961595

    Figure Lengend Snippet: Logistic regressions result for taxa within the composite and elevated depressive symptoms. Significant positive associations between P. nigrescens , F. nucleatum subsp. vincentii , F. nucleatum subsp. animalis ATCC 51191 , F. nucleatum subsp. animalis , and elevated depressive symptoms ( N = 39), compared to minimal depressive symptoms ( N = 397), both in unadjusted and adjusted models (all p < 0.05). No evidence of associations with the other species. Adjusted models control for confounders: age, SES, diet, lifestyle risk, obesity, and hypertension. Error bars represent 95% CI. 95% CI, 95% confidence interval; OR, odds ratio estimate; SE, standard error; SES, socioconomic status. Significant p -values ( p < 0.05) are in bold.

    Article Snippet: K. pneumoniae species were not included as no OTU assigned to them was detected; similarly, we did not include A. actinomycetemcomitans species and F. nucleatum subsp. fusiforme ATCC 51190 species as these were poorly detected in the samples and not positively correlated with the composite (details in Supporting Information Table ; correlation matrix in Supporting Information Figure ).

    Techniques: Control

    Comparisons of planktonic (p) and biofilm-grown (b) F . nucleatum (FN) stimulating antimicrobial responses in human neutrophils. (A–C) Quantification of overall ROS released shown as area under the curve (AUC). (D) Quantification of NET-DNA. (E–H) Quantification of cytokine and (I–J) antimicrobial enzyme release. Numbers of samples in each data set analysed are specified in <xref ref-type= Supplementary Table 1 . ns, not significant; *p<0.05; **p<0.01; ***p<0.001. (A, B, D, I, J) One-Way ANOVA with Tukey’s post hoc test, mean values and SD are shown. (C, E–H) Kruskal-Wallis test with Dunn’s post hoc test, values shown as median and interquartile range (IQR). " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fusobacterium nucleatum elicits subspecies-specific responses in human neutrophils

    doi: 10.3389/fcimb.2024.1449539

    Figure Lengend Snippet: Comparisons of planktonic (p) and biofilm-grown (b) F . nucleatum (FN) stimulating antimicrobial responses in human neutrophils. (A–C) Quantification of overall ROS released shown as area under the curve (AUC). (D) Quantification of NET-DNA. (E–H) Quantification of cytokine and (I–J) antimicrobial enzyme release. Numbers of samples in each data set analysed are specified in Supplementary Table 1 . ns, not significant; *p<0.05; **p<0.01; ***p<0.001. (A, B, D, I, J) One-Way ANOVA with Tukey’s post hoc test, mean values and SD are shown. (C, E–H) Kruskal-Wallis test with Dunn’s post hoc test, values shown as median and interquartile range (IQR).

    Article Snippet: F. nucleatum type strains, originally obtained from the American Tissue Culture Collection (ATCC) and maintained in the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) were used in this study: F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques:

    Quantification of peak ROS generation by neutrophils upon stimulation with F. nucleatum subspecies. Prefix “p” denotes planktonic subspecies, prefix “b” denotes biofilm-grown subspecies. Biofilm-grown FNP (bFNP) was unavailable due to the absence of biofilm formation. (A) Quantification of total ROS generation. All subspecies except pFNV are significantly higher than the negative control (GPBS), statistical significance detailed in a p-value matrix using “* (asterisk)” symbols. Significant differences between planktonic and biofilm-grown subspecies are indicated by “# (hash)” symbols. N=8 healthy adult volunteers. (B) Quantification of intracellular ROS generation. All subspecies except pFN23 are significantly higher than the negative control (GPBS), statistical significance detailed in a p-value matrix using “* (asterisk)” symbols. Significant difference between planktonic and biofilm-grown subspecies is indicated by “# (hash)” symbol. N=8 healthy adult volunteers. (C) Quantification of superoxide generation. N=6 healthy adult volunteers. Statistical significance detailed in a p-value matrix using “(asterisk)” symbols. All data are shown as mean values ±SD. Datasets were analysed using one-way ANOVA with Tukey’s post hoc test. ns – not significant; */# - p<0.05; **/## - p<0.01; *** - p<0.001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fusobacterium nucleatum elicits subspecies-specific responses in human neutrophils

    doi: 10.3389/fcimb.2024.1449539

    Figure Lengend Snippet: Quantification of peak ROS generation by neutrophils upon stimulation with F. nucleatum subspecies. Prefix “p” denotes planktonic subspecies, prefix “b” denotes biofilm-grown subspecies. Biofilm-grown FNP (bFNP) was unavailable due to the absence of biofilm formation. (A) Quantification of total ROS generation. All subspecies except pFNV are significantly higher than the negative control (GPBS), statistical significance detailed in a p-value matrix using “* (asterisk)” symbols. Significant differences between planktonic and biofilm-grown subspecies are indicated by “# (hash)” symbols. N=8 healthy adult volunteers. (B) Quantification of intracellular ROS generation. All subspecies except pFN23 are significantly higher than the negative control (GPBS), statistical significance detailed in a p-value matrix using “* (asterisk)” symbols. Significant difference between planktonic and biofilm-grown subspecies is indicated by “# (hash)” symbol. N=8 healthy adult volunteers. (C) Quantification of superoxide generation. N=6 healthy adult volunteers. Statistical significance detailed in a p-value matrix using “(asterisk)” symbols. All data are shown as mean values ±SD. Datasets were analysed using one-way ANOVA with Tukey’s post hoc test. ns – not significant; */# - p<0.05; **/## - p<0.01; *** - p<0.001.

    Article Snippet: F. nucleatum type strains, originally obtained from the American Tissue Culture Collection (ATCC) and maintained in the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) were used in this study: F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques: Negative Control

    Quantification of cytokine and antimicrobial enzyme release by neutrophils stimulated by F . nucleatum subspecies. Prefix “p” denotes planktonic subspecies, prefix “b” denotes biofilm-grown subspecies. (A) Quantification of IL-1β; LOQ=3.9 pg/ml. (B) Quantification of IL-6. Data were not normally distributed and are therefore shown as medians and interquartile ranges. LOQ=9.38 pg/ml. (C) Quantification of TNF-α. Statistical significance is detailed in a p-value matrix. LOQ=15.6 pg/ml. (D) Quantification of IL-8. Statistical significance is detailed in a p-value matrix. (E) Quantification of MMP-9 with no statistical difference among the tested subspecies. (F) Quantification of HNE with no statistical difference among the tested subspecies. Subspecies indicated with “$ (dollar)” symbols are significantly higher compared to the negative control (RPMI). Significant differences between planktonic and biofilm-grown subspecies are indicated by “# (hash)” symbols. Significant differences within the groups of planktonic and biofilm-grown subspecies are indicated by “* (asterisk)” symbols. Normally distributed data were analysed using one-way ANOVA followed by Tukey’s post hoc test. Non-normally distributed data were analysed using Kruskal-Wallis test followed by Dunn’s post hoc test. Assays were performed in technical duplicate, N=5 healthy adult volunteers. Data in A, C, D, E and F are shown as mean values ± SD. LOQ – limit of quantitation. */#/$ - p<0.05; **/##/$$ - p<0.01; ***/$$$ - p<0.001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fusobacterium nucleatum elicits subspecies-specific responses in human neutrophils

    doi: 10.3389/fcimb.2024.1449539

    Figure Lengend Snippet: Quantification of cytokine and antimicrobial enzyme release by neutrophils stimulated by F . nucleatum subspecies. Prefix “p” denotes planktonic subspecies, prefix “b” denotes biofilm-grown subspecies. (A) Quantification of IL-1β; LOQ=3.9 pg/ml. (B) Quantification of IL-6. Data were not normally distributed and are therefore shown as medians and interquartile ranges. LOQ=9.38 pg/ml. (C) Quantification of TNF-α. Statistical significance is detailed in a p-value matrix. LOQ=15.6 pg/ml. (D) Quantification of IL-8. Statistical significance is detailed in a p-value matrix. (E) Quantification of MMP-9 with no statistical difference among the tested subspecies. (F) Quantification of HNE with no statistical difference among the tested subspecies. Subspecies indicated with “$ (dollar)” symbols are significantly higher compared to the negative control (RPMI). Significant differences between planktonic and biofilm-grown subspecies are indicated by “# (hash)” symbols. Significant differences within the groups of planktonic and biofilm-grown subspecies are indicated by “* (asterisk)” symbols. Normally distributed data were analysed using one-way ANOVA followed by Tukey’s post hoc test. Non-normally distributed data were analysed using Kruskal-Wallis test followed by Dunn’s post hoc test. Assays were performed in technical duplicate, N=5 healthy adult volunteers. Data in A, C, D, E and F are shown as mean values ± SD. LOQ – limit of quantitation. */#/$ - p<0.05; **/##/$$ - p<0.01; ***/$$$ - p<0.001.

    Article Snippet: F. nucleatum type strains, originally obtained from the American Tissue Culture Collection (ATCC) and maintained in the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) were used in this study: F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques: Negative Control, Quantitation Assay

    Time-course release of neutrophil cytokines and enzymes over 18 hours stimulated by F . nucleatum subspecies. Prefix “p” denotes planktonic subspecies. (A) Release of IL-1β; LOQ=3.9 pg/ml. (B) Release of IL-6. No statistical analysis was performed at time points 0, 1, 2 and 4 because the concentration of IL-6 detected was 0. LOQ=9.38 pg/ml. (C) Release of TNF-α. LOQ=15.6 pg/ml. (D) Release of IL-8. (E) Release of MMP-9. (F) Release of HNE. All data are shown as mean values ± SD. Statistical significance was calculated at individual time points using one-way ANOVA followed by Tukey’s post hoc test. Statistical significance is not shown if all values within the same time point were below the LOQ. LOQ – limit of quantitation. ns, not significant; *p<0.05; **p<0.01; ***p<0.001. Significant differences are in comparison to subspecies indicated by colour-coding for each time point. N=3 healthy adult volunteers.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fusobacterium nucleatum elicits subspecies-specific responses in human neutrophils

    doi: 10.3389/fcimb.2024.1449539

    Figure Lengend Snippet: Time-course release of neutrophil cytokines and enzymes over 18 hours stimulated by F . nucleatum subspecies. Prefix “p” denotes planktonic subspecies. (A) Release of IL-1β; LOQ=3.9 pg/ml. (B) Release of IL-6. No statistical analysis was performed at time points 0, 1, 2 and 4 because the concentration of IL-6 detected was 0. LOQ=9.38 pg/ml. (C) Release of TNF-α. LOQ=15.6 pg/ml. (D) Release of IL-8. (E) Release of MMP-9. (F) Release of HNE. All data are shown as mean values ± SD. Statistical significance was calculated at individual time points using one-way ANOVA followed by Tukey’s post hoc test. Statistical significance is not shown if all values within the same time point were below the LOQ. LOQ – limit of quantitation. ns, not significant; *p<0.05; **p<0.01; ***p<0.001. Significant differences are in comparison to subspecies indicated by colour-coding for each time point. N=3 healthy adult volunteers.

    Article Snippet: F. nucleatum type strains, originally obtained from the American Tissue Culture Collection (ATCC) and maintained in the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) were used in this study: F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques: Concentration Assay, Quantitation Assay, Comparison

    Analysis of neutrophil necrosis triggered by F. nucleatum subspecies. Prefix “p” denotes planktonic subspecies, prefix “b” denotes biofilm-grown subspecies. (A) Start of necrosis. Test samples were significantly different from the negative control (cells only) and the positive control (LL-37). The level of significance was identical compared to both the negative and the positive control, therefore only one set of symbols is shown using “$ (dollar)” symbols. Significant differences within the groups of planktonic subspecies are indicated by “* (asterisk)” symbols. (B) Rate of necrosis. Negative control as well as test samples were significantly different from the positive control LL-37, as indicated by “$ (dollar)” symbols. Assays were performed in technical duplicate, N=4 healthy adult volunteers. All data are shown as mean values ± SD. One-way ANOVA was performed followed by Tukey’s post hoc test. *p<0.05; **p<0.01; $$$p<0.001. RFU, relative fluorescence units.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fusobacterium nucleatum elicits subspecies-specific responses in human neutrophils

    doi: 10.3389/fcimb.2024.1449539

    Figure Lengend Snippet: Analysis of neutrophil necrosis triggered by F. nucleatum subspecies. Prefix “p” denotes planktonic subspecies, prefix “b” denotes biofilm-grown subspecies. (A) Start of necrosis. Test samples were significantly different from the negative control (cells only) and the positive control (LL-37). The level of significance was identical compared to both the negative and the positive control, therefore only one set of symbols is shown using “$ (dollar)” symbols. Significant differences within the groups of planktonic subspecies are indicated by “* (asterisk)” symbols. (B) Rate of necrosis. Negative control as well as test samples were significantly different from the positive control LL-37, as indicated by “$ (dollar)” symbols. Assays were performed in technical duplicate, N=4 healthy adult volunteers. All data are shown as mean values ± SD. One-way ANOVA was performed followed by Tukey’s post hoc test. *p<0.05; **p<0.01; $$$p<0.001. RFU, relative fluorescence units.

    Article Snippet: F. nucleatum type strains, originally obtained from the American Tissue Culture Collection (ATCC) and maintained in the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) were used in this study: F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques: Negative Control, Positive Control, Fluorescence